Hotspot

trnE-UUC-trnY-GUA

LSC · 29604-29889 · 286 bp

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Pi 0.127
MarkerSeek score 60.60
Primer available yes
Label trnE-UUC-trnY-GUA

Feature Metadata

label_name
trnE-UUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
286
variable_sites
51
pi
0.127
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.21
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnY-GUA_p1 AAGTATGCCCCCATCGTCTA CCAGCGTAGACATATTGCCA 50.0 50.0 57.9 57.8 203-429 bp 1.0 89.3
trnE-UUC-trnY-GUA_p2 AAGTATGCCCCCATCGTCTA ACCAGCGTAGACATATTGCC 50.0 50.0 57.9 57.8 204-430 bp 1.0 89.3
trnE-UUC-trnY-GUA_p3 TCTCTTTCAAGGAGGCAACG CCAGCGTAGACATATTGCCA 50.0 50.0 57.8 57.8 168-394 bp 1.0 88.7
trnE-UUC-trnY-GUA_p4 TCTCTTTCAAGGAGGCAACG ACCAGCGTAGACATATTGCC 50.0 50.0 57.8 57.8 169-395 bp 1.0 88.7
trnE-UUC-trnY-GUA_p5 AAGTATGCCCCCATCGTCTA CGAATTTACAGTCCGTCCCC 50.0 55.0 57.9 58.4 182-408 bp 1.0 88.4