Hotspot

rps4-trnS-GGA

LSC · 15658-15885 · 228 bp

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Pi 0.0297
MarkerSeek score 72.40
Primer available yes
Label rps4-trnS-GGA

Feature Metadata

label_name
rps4-trnS-GGA
feature_type
igs
region
LSC
length_bp
228
variable_sites
24
pi
0.0297
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnS-GGA_p1 TGACTCTTCAGCCATTCCAA AGGCTTTTTGCTTACCGAGG 45.0 50.0 56.7 58.5 377-391 bp 1.0 75.2
rps4-trnS-GGA_p2 TTGACTCTTCAGCCATTCCA AGGCTTTTTGCTTACCGAGG 45.0 50.0 56.7 58.5 378-392 bp 1.0 75.2
rps4-trnS-GGA_p3 TGACTCTTCAGCCATTCCAA TTTTGCTTACCGAGGGTTCG 45.0 50.0 56.7 58.5 372-386 bp 1.0 75.2
rps4-trnS-GGA_p4 TTGACTCTTCAGCCATTCCA TTTTGCTTACCGAGGGTTCG 45.0 50.0 56.7 58.5 373-387 bp 1.0 75.1
rps4-trnS-GGA_p5 TTGACTCTTCAGCCATTCCAA AGGCTTTTTGCTTACCGAGG 42.9 50.0 57.5 58.5 378-392 bp 1.0 73.2