Hotspot

ndhJ-trnF-GAA

LSC · 12292-12892 · 601 bp

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Pi 0.0367
MarkerSeek score 80.10
Primer available yes
Label ndhJ-trnF-GAA

Feature Metadata

label_name
ndhJ-trnF-GAA
feature_type
igs
region
LSC
length_bp
601
variable_sites
74
pi
0.0367
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-trnF-GAA_p1 AATGCCCGAAAGCTGGATAG CCTCGTGTCACCAGTTCAAA 50.0 50.0 58.0 58.1 708-776 bp 1.0 91.3
ndhJ-trnF-GAA_p2 TTTAATGCCCGAAAGCTGGA CCTCGTGTCACCAGTTCAAA 45.0 50.0 57.8 58.1 711-779 bp 1.0 89.8
ndhJ-trnF-GAA_p3 AATGCCCGAAAGCTGGATAG AATCCTCGTGTCACCAGTTC 50.0 50.0 58.0 57.5 711-779 bp 1.0 88.0
ndhJ-trnF-GAA_p4 TTAATGCCCGAAAGCTGGAT CCTCGTGTCACCAGTTCAAA 45.0 50.0 57.6 58.1 710-778 bp 1.0 88.0
ndhJ-trnF-GAA_p5 TTTAATGCCCGAAAGCTGGA AATCCTCGTGTCACCAGTTC 45.0 50.0 57.8 57.5 714-782 bp 1.0 86.6