Hotspot

trnH-GUG-psbA

LSC · 114-391 · 278 bp

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Pi 0.0552
MarkerSeek score 73.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
278
variable_sites
42
pi
0.0552
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA CTGACGGTATGCCTCTAGGA 50.0 55.0 57.9 58.0 866-914 bp 1.0 84.9
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CTGACGGTATGCCTCTAGGA 55.0 55.0 58.1 58.0 852-900 bp 0.923077 81.7
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG TCTGACGGTATGCCTCTAGG 55.0 55.0 58.1 58.0 853-901 bp 0.923077 81.7
trnH-GUG-psbA_p4 TTTTTGGTATGGGCGAACGA CTGACGGTATGCCTCTAGGA 45.0 55.0 58.1 58.0 921-930 bp 0.307692 54.5
trnH-GUG-psbA_p5 TTTTTGGTATGGGCGAACGA TCTGACGGTATGCCTCTAGG 45.0 55.0 58.1 58.0 922-931 bp 0.307692 54.5