Hotspot

ycf1

SSC · 128623-134187 · 5565 bp

Back to Loropetalum

Pi 0.000359

MarkerSeek score 52.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5565
variable_sites: 2
pi: 0.000359
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTTCCACGATAGGGTCCAT	CGTATCCACCTAGCTTGTCA	50.0	50.0	58.1	56.8	290 bp	1.0	69.3
ycf1_p2	TGTTCCACGATAGGGTCCAT	ACGTATCCACCTAGCTTGTC	50.0	50.0	58.1	56.7	291 bp	1.0	69.3
ycf1_p3	TCCACGATAGGGTCCATTCA	CGTATCCACCTAGCTTGTCA	50.0	50.0	57.8	56.8	287 bp	1.0	68.9
ycf1_p4	TCCACGATAGGGTCCATTCA	ACGTATCCACCTAGCTTGTC	50.0	50.0	57.8	56.7	288 bp	1.0	68.9
ycf1_p5	TTGTTCCACGATAGGGTCCA	CGTATCCACCTAGCTTGTCA	50.0	50.0	58.4	56.8	291 bp	1.0	67.5