Hotspot

petB

LSC · 78766-80206 · 1441 bp

Back to Loropetalum

Pi 0.000694

MarkerSeek score 59.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1441
variable_sites: 1
pi: 0.000694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGAACCACGAAGTCCTAGCT	ATTGTTCCTCCCCCAAATGA	50.0	45.0	58.1	56.7	1612 bp	1.0	68.9
petB_p2	AGAACCACGAAGTCCTAGCT	TTGTTCCTCCCCCAAATGAT	50.0	45.0	58.1	56.7	1611 bp	1.0	68.9
petB_p3	TCACAAGAACCACGAAGTCC	ATTGTTCCTCCCCCAAATGA	50.0	45.0	57.8	56.7	1617 bp	1.0	67.6
petB_p4	TCACAAGAACCACGAAGTCC	TTGTTCCTCCCCCAAATGAT	50.0	45.0	57.8	56.7	1616 bp	1.0	67.6
petB_p5	CACAAGAACCACGAAGTCCT	TTGTTCCTCCCCCAAATGAT	50.0	45.0	57.8	56.7	1615 bp	1.0	67.6