Hotspot

ndhD

SSC · 120354-121874 · 1521 bp

Back to Loropetalum

Pi 0.000657

MarkerSeek score 59.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 1
pi: 0.000657
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCATTGTACAACGAATGGTT	CGTTCCAGAAAACTTCACGC	35.0	50.0	53.1	58.0	1704-1705 bp	1.0	40.9
ndhD_p2	TCATTGTACAACGAATGGTT	GTTCCAGAAAACTTCACGCG	35.0	50.0	53.1	58.0	1703-1704 bp	1.0	40.9
ndhD_p3	TTCATTGTACAACGAATGGT	CGTTCCAGAAAACTTCACGC	35.0	50.0	53.1	58.0	1705-1706 bp	1.0	40.9
ndhD_p4	TTCATTGTACAACGAATGGT	GTTCCAGAAAACTTCACGCG	35.0	50.0	53.1	58.0	1704-1705 bp	1.0	40.9
ndhD_p5	TCATTGTACAACGAATGGTT	TCCAGAAAACTTCACGCGAA	35.0	45.0	53.1	58.1	1701-1702 bp	1.0	40.6