Hotspot

trnN-GUU-trnR-ACG

IRa · 134582-135170 · 589 bp

Back to Loropetalum

Pi 0.0017

MarkerSeek score 59.50

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 589
variable_sites: 1
pi: 0.0017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	730 bp	1.0	78.4
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	767 bp	1.0	77.8
trnN-GUU-trnR-ACG_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	771 bp	1.0	77.6
trnN-GUU-trnR-ACG_p4	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	694 bp	1.0	77.2
trnN-GUU-trnR-ACG_p5	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	681 bp	1.0	77.1