Hotspot

atpB-rbcL

LSC · 58139-58841 · 703 bp

Back to Loropetalum

Pi 0.00284

MarkerSeek score 60.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 2
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	GGTGACATAAGTCCCTCCCT	55.0	55.0	58.0	58.1	832 bp	1.0	78.9
atpB-rbcL_p2	GGGGGAAAAGCTACATCCAG	GGTGACATAAGTCCCTCCCT	55.0	55.0	57.9	58.1	845 bp	1.0	78.8
atpB-rbcL_p3	ATCCAGTACCGGACCAATGA	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.1	58.1	831 bp	1.0	78.2
atpB-rbcL_p4	TCCAGTACCGGACCAATGAT	GGTGACATAAGTCCCTCCCT	50.0	55.0	58.1	58.1	830 bp	1.0	78.2
atpB-rbcL_p5	AAGCTACATCCAGTACCGGA	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.8	58.1	838 bp	1.0	78.0