Hotspot

trnD-GUC-trnY-GUA

LSC · 33026-33451 · 426 bp

Back to Loropetalum

Pi 0.00235

MarkerSeek score 56.50

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 1
pi: 0.00235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TGTTTATTGGATCCGTCGGG	AATTGCTTCTTCCTGGGTCG	50.0	50.0	57.7	58.2	614-615 bp	1.0	76.2
trnD-GUC-trnY-GUA_p2	TGTTTATTGGATCCGTCGGG	TGGCAATATGTCTACGCTGG	50.0	50.0	57.7	57.8	556-557 bp	1.0	75.7
trnD-GUC-trnY-GUA_p3	TGTTTATTGGATCCGTCGGG	GGCAATATGTCTACGCTGGT	50.0	50.0	57.7	57.8	555-556 bp	1.0	75.7
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	AATTGCTTCTTCCTGGGTCG	50.0	50.0	58.5	58.2	554-555 bp	1.0	74.9
trnD-GUC-trnY-GUA_p5	TGTTTATTGGATCCGTCGGG	GGGGACGGACTGTAAATTCG	50.0	55.0	57.7	58.4	577-578 bp	1.0	74.8