Hotspot

matK-trnK-UUU

LSC · 3580-4282 · 703 bp

Back to Loropetalum

Pi 0

MarkerSeek score 59.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GGAAGTCATGTTGCCGAGAT	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.0	58.2	780-782 bp	1.0	78.6
matK-trnK-UUU_p2	GGAAGTCATGTTGCCGAGAT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.0	58.2	779-781 bp	1.0	78.6
matK-trnK-UUU_p3	GGAAGTCATGTTGCCGAGAT	TTGCGTCGTACAATCCAGTC	50.0	50.0	58.0	58.3	872-874 bp	1.0	77.6
matK-trnK-UUU_p4	GGAAGTCATGTTGCCGAGAT	ATTGCGTCGTACAATCCAGT	50.0	45.0	58.0	57.6	873-875 bp	1.0	76.9
matK-trnK-UUU_p5	GGAAGTCATGTTGCCGAGAT	TTGGGTTGCTAACTCAACGG	50.0	50.0	58.0	58.4	795-797 bp	1.0	76.8