Hotspot

psbA

LSC · 277-1338 · 1062 bp

Back to Loranthus

Pi 0.00647

MarkerSeek score 35.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 14
pi: 0.00647
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGAACTCGTATACACTAGTAAAGA	CGTGCTTGGGAGTCTTTGAT	33.3	50.0	54.4	58.2	1132 bp	1.0	41.3
psbA_p2	AGAACTCGTATACACTAGTAAAGA	AGAGAGTTTTCGTGCTTGGG	33.3	50.0	54.4	58.1	1142 bp	0.833333	34.5
psbA_p3	AGAACTCGTATACACTAGTAAAGA	AGAGTTTTCGTGCTTGGGAG	33.3	50.0	54.4	58.1	1140 bp	0.833333	34.5
psbA_p4	AGAACTCGTATACACTAGTAAAGA	GAGAGTTTTCGTGCTTGGGA	33.3	50.0	54.4	58.1	1141 bp	0.833333	34.5
psbA_p5	AGAACTCGTATACACTAGTAAAGA	TTTCGTGCTTGGGAGTCTTT	33.3	45.0	54.4	57.7	1135 bp	0.833333	34.5