Hotspot

ndhB-trnL-CAA

IRa · 113895-114650 · 756 bp

Back to Loranthus

Pi 0.0067

MarkerSeek score 64.50

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 756
variable_sites: 12
pi: 0.0067
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TCCGTCGACAAAAGGATCAC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	801-836 bp	1.0	84.5
ndhB-trnL-CAA_p2	TCCGTCGACAAAAGGATCAC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	799-834 bp	1.0	84.5
ndhB-trnL-CAA_p3	TCCGTCGACAAAAGGATCAC	TACCATTTCACCACCAAGGC	50.0	50.0	57.9	58.1	789-824 bp	1.0	83.9
ndhB-trnL-CAA_p4	TCCGTCGACAAAAGGATCAC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	796-831 bp	1.0	83.0
ndhB-trnL-CAA_p5	TCCGTCGACAAAAGGATCAC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	800-835 bp	1.0	82.4