Hotspot

rpl16-rps3

LSC · 68157-68748 · 592 bp

Back to Loranthus

Pi 0.0255

MarkerSeek score 64.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 33
pi: 0.0255
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TCTTCTCTACTACAGAACCGT	TGTTCCTATCCAACTCGAACT	42.9	42.9	55.2	56.4	600-732 bp	1.0	47.9
rpl16-rps3_p2	TTCTTCTCTACTACAGAACCGT	TGTTCCTATCCAACTCGAACT	40.9	42.9	56.0	56.4	601-733 bp	1.0	47.9
rpl16-rps3_p3	TGTTTCTTCTCTACTACAGAACCG	TGTTCCTATCCAACTCGAACT	41.7	42.9	58.0	56.4	604-736 bp	1.0	47.9
rpl16-rps3_p4	GTTTCTTCTCTACTACAGAACCGT	TGTTCCTATCCAACTCGAACT	41.7	42.9	58.0	56.4	603-735 bp	1.0	47.9
rpl16-rps3_p5	TCTCTACTACAGAACCGTACA	TGTTCCTATCCAACTCGAACT	42.9	42.9	55.0	56.4	597-729 bp	1.0	47.9