Hotspot

trnP-UGG-psaJ

LSC · 53936-54179 · 244 bp

Back to Loranthus

Pi 0.0287

MarkerSeek score 64.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 16
pi: 0.0287
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TACCAAACTGCGCTACATCC	GCCGCGGAGAGATAAGTTTT	50.0	50.0	58.0	58.3	267-389 bp	1.0	85.1
trnP-UGG-psaJ_p2	TGTACCCAAAACAAATGCGC	GCCGCGGAGAGATAAGTTTT	45.0	50.0	57.9	58.3	287-409 bp	1.0	83.9
trnP-UGG-psaJ_p3	TACCAAACTGCGCTACATCC	ATAGACCCGCCAAAGATCCA	50.0	50.0	58.0	58.5	311-433 bp	1.0	83.4
trnP-UGG-psaJ_p4	AGTGATAGGCAGGGATGACA	GCCGCGGAGAGATAAGTTTT	50.0	50.0	57.8	58.3	328-450 bp	1.0	83.2
trnP-UGG-psaJ_p5	TAGGCAGGGATGACAGGATT	GCCGCGGAGAGATAAGTTTT	50.0	50.0	57.8	58.3	323-445 bp	1.0	83.1