Hotspot

petL-petG

LSC · 53222-53407 · 186 bp

Back to Loranthus

Pi 0.0262

MarkerSeek score 62.60

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 10
pi: 0.0262
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	CGGCTTTAACTATAACCTCAGC	CGACCGCTACGTCTGTATTG	45.5	55.0	56.9	58.5	334-349 bp	1.0	56.9
petL-petG_p2	CGGCTTTAACTATAACCTCAGC	GACCGCTACGTCTGTATTGT	45.5	50.0	56.9	57.4	333-348 bp	1.0	56.7
petL-petG_p3	ACCTCAGCTTTATTTGTTGGT	CGACCGCTACGTCTGTATTG	38.1	55.0	55.7	58.5	320-335 bp	1.0	55.0
petL-petG_p4	CGGCTTTAACTATAACCTCAGCT	CGACCGCTACGTCTGTATTG	43.5	55.0	58.3	58.5	334-349 bp	1.0	54.9
petL-petG_p5	CGGCTTTAACTATAACCTCAGCT	GACCGCTACGTCTGTATTGT	43.5	50.0	58.3	57.4	333-348 bp	1.0	54.7