Hotspot

trnT-UGU-trnL-UAA

LSC · 39802-40493 · 692 bp

Back to Loranthus

Pi 0.0241
MarkerSeek score 65.20
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
692
variable_sites
38
pi
0.0241
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 711-785 bp 1.0 86.9
trnT-UGU-trnL-UAA_p2 GACCGTCGCATTACAAATGC ACCAATTTCGCCATATCCCC 50.0 50.0 58.2 58.0 729-803 bp 1.0 86.4
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 719-793 bp 1.0 86.4
trnT-UGU-trnL-UAA_p4 GACCGTCGCATTACAAATGC TAGCGTCTACCAATTTCGCC 50.0 50.0 58.2 58.1 737-811 bp 1.0 85.8
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 725-799 bp 1.0 85.0