Hotspot

psbM-trnD-GUC

LSC · 24233-24867 · 635 bp

Back to Loranthus

Pi 0.0227

MarkerSeek score 64.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 31
pi: 0.0227
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTGACTAGCCGTTTTTACGT	TTCAACTGGTTAGAGCACCG	40.0	50.0	55.4	57.8	691-835 bp	1.0	64.7
psbM-trnD-GUC_p2	AGCCGTTTTTACGTAAAGGA	TTCAACTGGTTAGAGCACCG	40.0	50.0	55.3	57.8	685-829 bp	1.0	64.4
psbM-trnD-GUC_p3	TTTGACTAGCCGTTTTTACGT	TTCAACTGGTTAGAGCACCG	38.1	50.0	56.1	57.8	692-836 bp	1.0	63.0
psbM-trnD-GUC_p4	AGCCGTTTTTACGTAAAGGAT	TTCAACTGGTTAGAGCACCG	38.1	50.0	55.9	57.8	685-829 bp	1.0	61.0
psbM-trnD-GUC_p5	ACTAGCCGTTTTTACGTAAAGGA	TTCAACTGGTTAGAGCACCG	39.1	50.0	58.1	57.8	688-832 bp	1.0	60.9