Hotspot

atpI-rps2

LSC · 10693-10908 · 216 bp

Back to Loranthus

Pi 0.017

MarkerSeek score 61.90

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 216
variable_sites: 7
pi: 0.017
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	TGAGCATGGACTTGGAAACC	ATTTCGATTCCGGCGAATGA	50.0	45.0	58.1	58.1	426-437 bp	1.0	83.1
atpI-rps2_p2	TGAGCATGGACTTGGAAACC	TTTCGATTCCGGCGAATGAT	50.0	45.0	58.1	58.1	425-436 bp	1.0	83.1
atpI-rps2_p3	TGAGCATGGACTTGGAAACC	GGATATTTCGATTCCGGCGA	50.0	50.0	58.1	57.9	430-441 bp	1.0	82.9
atpI-rps2_p4	GAGCATGGACTTGGAAACCT	ATTTCGATTCCGGCGAATGA	50.0	45.0	57.8	58.1	425-436 bp	1.0	82.2
atpI-rps2_p5	GAGCATGGACTTGGAAACCT	TTTCGATTCCGGCGAATGAT	50.0	45.0	57.8	58.1	424-435 bp	1.0	82.2