Hotspot

trnH-psbA

LSC · 44-276 · 233 bp

Back to Loranthus

Pi 0.0569

MarkerSeek score 76.60

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 26
pi: 0.0569
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	CAATCCACTGCCTTGATCCA	GCCCGTAGTAGGTATCTGGT	50.0	55.0	57.9	58.0	480-497 bp	0.833333	79.9
trnH-psbA_p2	AATCCACTGCCTTGATCCAC	GCCCGTAGTAGGTATCTGGT	50.0	55.0	57.9	58.0	479-496 bp	0.833333	79.9
trnH-psbA_p3	CAATCCACTGCCTTGATCCA	TGGCCCGTAGTAGGTATCTG	50.0	55.0	57.9	58.0	482-499 bp	0.833333	79.8
trnH-psbA_p4	ACAATCCACTGCCTTGATCC	GCCCGTAGTAGGTATCTGGT	50.0	55.0	57.9	58.0	481-498 bp	0.833333	79.8
trnH-psbA_p5	CAATCCACTGCCTTGATCCA	GTTGATAGTCAAGGTCGCGT	50.0	50.0	57.9	58.0	408-412 bp	0.5	63.2