primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	trnH-psbA	1	CAATCCACTGCCTTGATCCA	GCCCGTAGTAGGTATCTGGT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.140744	44	276	480-497	0.833333	79.9
trnH-psbA_p2	trnH-psbA	2	AATCCACTGCCTTGATCCAC	GCCCGTAGTAGGTATCTGGT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.142251	44	276	479-496	0.833333	79.9
trnH-psbA_p3	trnH-psbA	3	CAATCCACTGCCTTGATCCA	TGGCCCGTAGTAGGTATCTG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.142160	44	276	482-499	0.833333	79.8
trnH-psbA_p4	trnH-psbA	4	ACAATCCACTGCCTTGATCC	GCCCGTAGTAGGTATCTGGT	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.142251	44	276	481-498	0.833333	79.8
trnH-psbA_p5	trnH-psbA	5	CAATCCACTGCCTTGATCCA	GTTGATAGTCAAGGTCGCGT	20	20	50.0	50.0	57.9	58.0	0.0	5.8	0.141112	44	276	408-412	0.500000	63.2
psbA_p1	psbA	1	AGAACTCGTATACACTAGTAAAGA	CGTGCTTGGGAGTCTTTGAT	24	20	33.3	50.0	54.4	58.2	3.3	0.0	7.813222	277	1338	1132-1132	1.000000	41.3
psbA_p2	psbA	2	AGAACTCGTATACACTAGTAAAGA	AGAGAGTTTTCGTGCTTGGG	24	20	33.3	50.0	54.4	58.1	3.3	0.0	7.742500	277	1338	1142-1142	0.833333	34.5
psbA_p3	psbA	3	AGAACTCGTATACACTAGTAAAGA	AGAGTTTTCGTGCTTGGGAG	24	20	33.3	50.0	54.4	58.1	3.3	0.0	7.742500	277	1338	1140-1140	0.833333	34.5
psbA_p4	psbA	4	AGAACTCGTATACACTAGTAAAGA	GAGAGTTTTCGTGCTTGGGA	24	20	33.3	50.0	54.4	58.1	3.3	0.0	7.747782	277	1338	1141-1141	0.833333	34.5
psbA_p5	psbA	5	AGAACTCGTATACACTAGTAAAGA	TTTCGTGCTTGGGAGTCTTT	24	20	33.3	45.0	54.4	57.7	3.3	0.0	7.968581	277	1338	1135-1135	0.833333	34.5
atpI-rps2_p1	atpI-rps2	1	TGAGCATGGACTTGGAAACC	ATTTCGATTCCGGCGAATGA	20	20	50.0	45.0	58.1	58.1	0.0	9.3	0.142196	10693	10908	426-437	1.000000	83.1
atpI-rps2_p2	atpI-rps2	2	TGAGCATGGACTTGGAAACC	TTTCGATTCCGGCGAATGAT	20	20	50.0	45.0	58.1	58.1	0.0	9.3	0.142196	10693	10908	425-436	1.000000	83.1
atpI-rps2_p3	atpI-rps2	3	TGAGCATGGACTTGGAAACC	GGATATTTCGATTCCGGCGA	20	20	50.0	50.0	58.1	57.9	0.0	9.3	0.164847	10693	10908	430-441	1.000000	82.9
atpI-rps2_p4	atpI-rps2	4	GAGCATGGACTTGGAAACCT	ATTTCGATTCCGGCGAATGA	20	20	50.0	45.0	57.8	58.1	0.0	9.3	0.258472	10693	10908	425-436	1.000000	82.2
atpI-rps2_p5	atpI-rps2	5	GAGCATGGACTTGGAAACCT	TTTCGATTCCGGCGAATGAT	20	20	50.0	45.0	57.8	58.1	0.0	9.3	0.258472	10693	10908	424-435	1.000000	82.2
petN-psbM_p1	petN-psbM	1	GTATGGGGGAGAAGTGGACT	ACGTAAAAACGGCTAGTCAA	20	20	55.0	40.0	58.1	55.4	0.0	2.8	2.768788	23267	24118	595-915	1.000000	64.8
petN-psbM_p2	petN-psbM	2	AGTATGGGGGAGAAGTGGAC	ACGTAAAAACGGCTAGTCAA	20	20	55.0	40.0	58.1	55.4	0.0	2.8	2.768788	23267	24118	596-916	1.000000	64.7
petN-psbM_p3	petN-psbM	3	AGTATGGGGGAGAAGTGGAC	TCCTTTACGTAAAAACGGCT	20	20	55.0	40.0	58.1	55.3	0.0	20.5	2.799875	23267	24118	602-922	1.000000	64.5
petN-psbM_p4	petN-psbM	4	GTATGGGGGAGAAGTGGACT	TCCTTTACGTAAAAACGGCT	20	20	55.0	40.0	58.1	55.3	0.0	20.5	2.799875	23267	24118	601-921	1.000000	64.5
petN-psbM_p5	petN-psbM	5	TATGGGGGAGAAGTGGACTC	ACGTAAAAACGGCTAGTCAA	20	20	55.0	40.0	57.8	55.4	0.0	2.8	2.825976	23267	24118	594-914	1.000000	64.3
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TTGACTAGCCGTTTTTACGT	TTCAACTGGTTAGAGCACCG	20	20	40.0	50.0	55.4	57.8	0.0	0.0	2.813547	24233	24867	691-835	1.000000	64.7
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGCCGTTTTTACGTAAAGGA	TTCAACTGGTTAGAGCACCG	20	20	40.0	50.0	55.3	57.8	22.4	0.0	2.844634	24233	24867	685-829	1.000000	64.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TTTGACTAGCCGTTTTTACGT	TTCAACTGGTTAGAGCACCG	21	20	38.1	50.0	56.1	57.8	0.0	0.0	3.024094	24233	24867	692-836	1.000000	63.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGCCGTTTTTACGTAAAGGAT	TTCAACTGGTTAGAGCACCG	21	20	38.1	50.0	55.9	57.8	22.4	0.0	3.269050	24233	24867	685-829	1.000000	61.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	ACTAGCCGTTTTTACGTAAAGGA	TTCAACTGGTTAGAGCACCG	23	20	39.1	50.0	58.1	57.8	22.4	0.0	3.281898	24233	24867	688-832	1.000000	60.9
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	TCGTAGGTCATTTGTGGCAC	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.2	58.1	11.6	0.0	0.255341	29235	29401	354-355	1.000000	80.4
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	TCGTAGGTCATTTGTGGCAC	GGGTTCGAATCCCTCTCTCT	20	20	50.0	55.0	58.2	58.2	11.6	17.2	0.413595	29235	29401	300-301	1.000000	79.6
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	GCAGCAGGATTTGAAAAGGG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.433848	29235	29401	313-314	1.000000	79.3
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.434339	29235	29401	317-318	1.000000	79.3
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	57.6	58.1	33.4	0.0	0.502562	29235	29401	316-317	1.000000	78.8
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TGCCTCCCTTTCTCTTGAAG	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.961472	37953	38536	733-763	1.000000	80.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	GCCTCCCTTTCTCTTGAAGT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.963464	37953	38536	732-762	1.000000	80.4
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TGCCTCCCTTTCTCTTGAAG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.1	57.8	0.0	5.6	1.059773	37953	38536	704-734	1.000000	79.7
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	GCCTCCCTTTCTCTTGAAGT	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.1	57.8	0.0	5.6	1.061765	37953	38536	703-733	1.000000	79.7
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	GCCTCCCTTTCTCTTGAAGTT	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	57.9	58.1	0.0	0.0	1.241916	37953	38536	732-762	1.000000	78.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	39802	40493	711-785	1.000000	86.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GACCGTCGCATTACAAATGC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.2	58.0	13.4	0.0	0.194427	39802	40493	729-803	1.000000	86.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	39802	40493	719-793	1.000000	86.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GACCGTCGCATTACAAATGC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.2	58.1	13.4	0.0	0.259236	39802	40493	737-811	1.000000	85.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	39802	40493	725-799	1.000000	85.0
atpB-rbcL_p1	atpB-rbcL	1	GCTACATCCAATACCGGACC	AACACCGGCTTTGAATCCAA	20	20	55.0	45.0	57.8	58.3	9.5	0.0	0.466813	43855	44671	961-979	1.000000	82.1
atpB-rbcL_p2	atpB-rbcL	2	GCTACATCCAATACCGGACC	TAACACCGGCTTTGAATCCA	20	20	55.0	45.0	57.8	57.4	9.5	0.0	0.735733	43855	44671	962-980	1.000000	79.9
atpB-rbcL_p3	atpB-rbcL	3	GGGGGAAAAGCTACATCCAA	AACACCGGCTTTGAATCCAA	20	20	50.0	45.0	57.5	58.3	12.8	0.0	0.826599	43855	44671	970-988	1.000000	79.2
atpB-rbcL_p4	atpB-rbcL	4	TTTTCAGGCACAGAAACCCC	AACACCGGCTTTGAATCCAA	20	20	50.0	45.0	58.6	58.3	0.3	0.0	0.892525	43855	44671	910-928	1.000000	78.8
atpB-rbcL_p5	atpB-rbcL	5	GCTACATCCAATACCGGACC	CCGGCTTTGAATCCAACACT	20	20	55.0	50.0	57.8	58.8	9.5	0.0	0.917752	43855	44671	957-975	1.000000	78.5
ycf4-cemA_p1	ycf4-cemA	1	AAGTCGCGGAATTGGCTTAT	GAGAAAGATCCACAAGGGCA	20	20	45.0	50.0	58.0	57.5	6.1	0.0	0.535285	49238	49372	248-260	1.000000	84.4
ycf4-cemA_p2	ycf4-cemA	2	AGTCGCGGAATTGGCTTATT	GAGAAAGATCCACAAGGGCA	20	20	45.0	50.0	58.0	57.5	6.1	0.0	0.535285	49238	49372	247-259	1.000000	84.4
ycf4-cemA_p3	ycf4-cemA	3	AAAGTCGCGGAATTGGCTTA	GAGAAAGATCCACAAGGGCA	20	20	45.0	50.0	58.2	57.5	6.1	0.0	0.680824	49238	49372	249-261	1.000000	83.2
ycf4-cemA_p4	ycf4-cemA	4	AAGTCGCGGAATTGGCTTAT	AGAGAAAGATCCACAAGGGC	20	20	45.0	50.0	58.0	57.2	6.1	0.0	0.835564	49238	49372	249-261	1.000000	82.0
ycf4-cemA_p5	ycf4-cemA	5	AGTCGCGGAATTGGCTTATT	AGAGAAAGATCCACAAGGGC	20	20	45.0	50.0	58.0	57.2	6.1	0.0	0.835564	49238	49372	248-260	1.000000	82.0
petL-petG_p1	petL-petG	1	CGGCTTTAACTATAACCTCAGC	CGACCGCTACGTCTGTATTG	22	20	45.5	55.0	56.9	58.5	0.0	0.0	3.592271	53222	53407	334-349	1.000000	56.9
petL-petG_p2	petL-petG	2	CGGCTTTAACTATAACCTCAGC	GACCGCTACGTCTGTATTGT	22	20	45.5	50.0	56.9	57.4	0.0	0.0	3.612611	53222	53407	333-348	1.000000	56.7
petL-petG_p3	petL-petG	3	ACCTCAGCTTTATTTGTTGGT	CGACCGCTACGTCTGTATTG	21	20	38.1	55.0	55.7	58.5	0.0	0.0	3.859543	53222	53407	320-335	1.000000	55.0
petL-petG_p4	petL-petG	4	CGGCTTTAACTATAACCTCAGCT	CGACCGCTACGTCTGTATTG	23	20	43.5	55.0	58.3	58.5	0.0	0.0	3.841856	53222	53407	334-349	1.000000	54.9
petL-petG_p5	petL-petG	5	CGGCTTTAACTATAACCTCAGCT	GACCGCTACGTCTGTATTGT	23	20	43.5	50.0	58.3	57.4	0.0	0.0	3.862195	53222	53407	333-348	1.000000	54.7
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	TACCAAACTGCGCTACATCC	GCCGCGGAGAGATAAGTTTT	20	20	50.0	50.0	58.0	58.3	9.7	33.5	0.365391	53936	54179	267-389	1.000000	85.1
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	TGTACCCAAAACAAATGCGC	GCCGCGGAGAGATAAGTTTT	20	20	45.0	50.0	57.9	58.3	0.0	33.5	0.488384	53936	54179	287-409	1.000000	83.9
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	TACCAAACTGCGCTACATCC	ATAGACCCGCCAAAGATCCA	20	20	50.0	50.0	58.0	58.5	9.7	0.0	0.511402	53936	54179	311-433	1.000000	83.4
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	AGTGATAGGCAGGGATGACA	GCCGCGGAGAGATAAGTTTT	20	20	50.0	50.0	57.8	58.3	0.0	33.5	0.527794	53936	54179	328-450	1.000000	83.2
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TAGGCAGGGATGACAGGATT	GCCGCGGAGAGATAAGTTTT	20	20	50.0	50.0	57.8	58.3	0.0	33.5	0.543591	53936	54179	323-445	1.000000	83.1
psbT-psbN_p1	psbT-psbN	1	AACCGCCTAAAGTTCCAACA	GCCCTCTCAACAACTACGAG	20	20	45.0	55.0	57.6	58.0	0.0	0.0	0.354037	60963	61029	148-154	1.000000	82.0
psbT-psbN_p2	psbT-psbN	2	ACCGCCTAAAGTTCCAACAA	GCCCTCTCAACAACTACGAG	20	20	45.0	55.0	57.6	58.0	0.0	0.0	0.354037	60963	61029	147-153	1.000000	82.0
psbT-psbN_p3	psbT-psbN	3	AACCGCCTAAAGTTCCAACA	AGATCCATTCGACGAACACG	20	20	45.0	50.0	57.6	58.1	0.0	13.4	0.442330	60963	61029	130-136	1.000000	81.9
psbT-psbN_p4	psbT-psbN	4	ACCGCCTAAAGTTCCAACAA	AGATCCATTCGACGAACACG	20	20	45.0	50.0	57.6	58.1	0.0	13.4	0.442330	60963	61029	129-135	1.000000	81.9
psbT-psbN_p5	psbT-psbN	5	TCGAGAACCGCCTAAAGTTC	GCCCTCTCAACAACTACGAG	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.361270	60963	61029	153-159	1.000000	81.7
rpl16-rps3_p1	rpl16-rps3	1	TCTTCTCTACTACAGAACCGT	TGTTCCTATCCAACTCGAACT	21	21	42.9	42.9	55.2	56.4	0.0	0.0	6.390521	68157	68748	600-732	1.000000	47.9
rpl16-rps3_p2	rpl16-rps3	2	TTCTTCTCTACTACAGAACCGT	TGTTCCTATCCAACTCGAACT	22	21	40.9	42.9	56.0	56.4	0.0	0.0	6.613969	68157	68748	601-733	1.000000	47.9
rpl16-rps3_p3	rpl16-rps3	3	TGTTTCTTCTCTACTACAGAACCG	TGTTCCTATCCAACTCGAACT	24	21	41.7	42.9	58.0	56.4	0.0	0.0	6.619051	68157	68748	604-736	1.000000	47.9
rpl16-rps3_p4	rpl16-rps3	4	GTTTCTTCTCTACTACAGAACCGT	TGTTCCTATCCAACTCGAACT	24	21	41.7	42.9	58.0	56.4	0.0	0.0	6.620232	68157	68748	603-735	1.000000	47.9
rpl16-rps3_p5	rpl16-rps3	5	TCTCTACTACAGAACCGTACA	TGTTCCTATCCAACTCGAACT	21	21	42.9	42.9	55.0	56.4	0.0	0.0	6.656585	68157	68748	597-729	1.000000	47.9
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	TCCGTCGACAAAAGGATCAC	20	20	50.0	50.0	58.0	57.9	5.8	17.2	0.082377	80207	80962	801-836	1.000000	84.5
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	TCCGTCGACAAAAGGATCAC	20	20	50.0	50.0	58.0	57.9	5.8	17.2	0.083748	80207	80962	799-834	1.000000	84.5
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	TACCATTTCACCACCAAGGC	TCCGTCGACAAAAGGATCAC	20	20	50.0	50.0	58.1	57.9	0.0	17.2	0.156675	80207	80962	789-824	1.000000	83.9
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	GCGTGTCTACCATTTCACCA	TCCGTCGACAAAAGGATCAC	20	20	50.0	50.0	58.2	57.9	0.0	17.2	0.271213	80207	80962	796-831	1.000000	83.0
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	TCTCGCGTGTCTACCATTTC	TCCGTCGACAAAAGGATCAC	20	20	50.0	50.0	57.7	57.9	5.8	17.2	0.347916	80207	80962	800-835	1.000000	82.4
ycf1_p1	ycf1	1	TGAGCAAGCGGAATGGTATC	CCGATTAGCCGAGGATCATG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.048253	99245	99364	432-432	1.000000	84.6
ycf1_p2	ycf1	2	TGAGCAAGCGGAATGGTATC	CGATTAGCCGAGGATCATGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.048253	99245	99364	431-431	1.000000	84.6
ycf1_p3	ycf1	3	CAAGCGGAATGGTATCCCAA	CCGATTAGCCGAGGATCATG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.053146	99245	99364	428-428	0.666667	68.3
ycf1_p4	ycf1	4	CAAGCGGAATGGTATCCCAA	CGATTAGCCGAGGATCATGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.053146	99245	99364	427-427	0.666667	68.3
ycf1_p5	ycf1	5	GCGGAATGGTATCCCAAGTT	CGATTAGCCGAGGATCATGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.054594	99245	99364	424-424	0.666667	68.3
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	TCCGTCGACAAAAGGATCAC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.9	58.0	17.2	5.8	0.082377	113895	114650	801-836	1.000000	84.5
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	TCCGTCGACAAAAGGATCAC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.9	58.0	17.2	5.8	0.083748	113895	114650	799-834	1.000000	84.5
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	TCCGTCGACAAAAGGATCAC	TACCATTTCACCACCAAGGC	20	20	50.0	50.0	57.9	58.1	17.2	0.0	0.156675	113895	114650	789-824	1.000000	83.9
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	TCCGTCGACAAAAGGATCAC	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	57.9	58.2	17.2	0.0	0.271213	113895	114650	796-831	1.000000	83.0
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TCCGTCGACAAAAGGATCAC	TCTCGCGTGTCTACCATTTC	20	20	50.0	50.0	57.9	57.7	17.2	5.8	0.347916	113895	114650	800-835	1.000000	82.4