Hotspot

ycf80

Genome · 54470-55984 · 1515 bp

Back to Lophurella

Pi 0.081

MarkerSeek score 79.90

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1515
variable_sites: 262
pi: 0.081
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	GGGTTCAAATCCCACCAGTT	AGGCGACTTACCAGGACTAA	50.0	50.0	58.0	57.8	2190 bp	0.2	46.1
ycf80_p2	GGGTTCAAATCCCACCAGTT	TTAGGCGACTTACCAGGACT	50.0	50.0	58.0	57.8	2192 bp	0.2	46.1
ycf80_p3	ACTTTGGTGGTCGAAGGTTC	AGGCGACTTACCAGGACTAA	50.0	50.0	58.0	57.8	2655 bp	0.2	45.9
ycf80_p4	ACTTTGGTGGTCGAAGGTTC	TTAGGCGACTTACCAGGACT	50.0	50.0	58.0	57.8	2657 bp	0.2	45.9
ycf80_p5	GACTTTGGTGGTCGAAGGTT	AGGCGACTTACCAGGACTAA	50.0	50.0	58.0	57.8	2656 bp	0.2	45.9