Hotspot

ycf58

Genome · 67479-67892 · 414 bp

Back to Lophurella

Pi 0.13

MarkerSeek score 77.90

Primer available yes

Label ycf58

Feature Metadata

label_name: ycf58
feature_type: gene
region: Genome
length_bp: 414
variable_sites: 110
pi: 0.13
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf58_p1	GCATTAGCGCTCTCTTGTGA	ATCAGGTCGTCGTGCTAAAA	50.0	45.0	58.4	57.3	1614-1656 bp	0.6	63.4
ycf58_p2	GCATTAGCGCTCTCTTGTGA	TCAGGTCGTCGTGCTAAAAT	50.0	45.0	58.4	57.3	1613-1655 bp	0.6	63.4
ycf58_p3	GCATTAGCGCTCTCTTGTGA	ATTGAAATGTTAGCGCGTGG	50.0	45.0	58.4	57.5	1502 bp	0.2	40.8
ycf58_p4	GCATTAGCGCTCTCTTGTGA	TGAAATGTTAGCGCGTGGTT	50.0	45.0	58.4	58.8	1500 bp	0.2	39.0
ycf58_p5	GCATTAGCGCTCTCTTGTGA	TTGAAATGTTAGCGCGTGGT	50.0	45.0	58.4	58.8	1501 bp	0.2	39.0