Hotspot

tilS

Genome · 32352-33335 · 984 bp

Back to Lophurella

Pi 0.0734
MarkerSeek score 81.20
Primer available yes
Label tilS

Feature Metadata

label_name
tilS
feature_type
gene
region
Genome
length_bp
984
variable_sites
157
pi
0.0734
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tilS_p1 AGGTCTTGCTCGACACAAAG TATCAAGCATGAACAGCGCT 50.0 45.0 58.1 58.0 1823 bp 0.2 46.7
tilS_p2 GAAGGTCTTGCTCGACACAA TATCAAGCATGAACAGCGCT 50.0 45.0 58.1 58.0 1825 bp 0.2 46.7
tilS_p3 GGTCTTGCTCGACACAAAGA TATCAAGCATGAACAGCGCT 50.0 45.0 58.1 58.0 1822 bp 0.2 46.7
tilS_p4 TTGAAGGTCTTGCTCGACAC TATCAAGCATGAACAGCGCT 50.0 45.0 58.1 58.0 1827 bp 0.2 46.7
tilS_p5 AGGTCTTGCTCGACACAAAG AAGCATGAACAGCGCTTCTA 50.0 45.0 58.1 57.9 1819 bp 0.2 46.2