Hotspot

ilvH

Genome · 57374-57910 · 537 bp

Back to Lophurella

Pi 0.0507

MarkerSeek score 77.50

Primer available yes

Label ilvH

Feature Metadata

label_name: ilvH
feature_type: gene
region: Genome
length_bp: 537
variable_sites: 64
pi: 0.0507
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ilvH_p1	CTGCATTGTGCTGCTTCTTT	GATCGTGAGGGTTCAAGTCC	45.0	55.0	57.6	58.0	1303 bp	0.2	44.4
ilvH_p2	CTGCATTGTGCTGCTTCTTT	AGGCGGTATAGCTAAGTGGT	45.0	50.0	57.6	57.9	1443 bp	0.2	43.9
ilvH_p3	CTGCATTGTGCTGCTTCTTT	GGTAAGGCAGAGGATTGCAA	45.0	50.0	57.6	57.9	1426 bp	0.2	43.5
ilvH_p4	CTGCATTGTGCTGCTTCTTT	CCCTCCATCCCCATTGACTA	45.0	55.0	57.6	58.2	1285 bp	0.2	43.1
ilvH_p5	CTGCATTGTGCTGCTTCTTT	GCTAAGTGGTAAGGCAGAGG	45.0	55.0	57.6	57.7	1433 bp	0.2	42.0