Hotspot

dnaB

Genome · 146719-148536 · 1818 bp

Back to Lophurella

Pi 0.0829

MarkerSeek score 78.60

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1818
variable_sites: 329
pi: 0.0829
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TCAGTCCACTGCTCTACCAA	TCATAAATGTTGCCCGTGGA	50.0	45.0	58.0	57.5	2318-2325 bp	0.8	75.9
dnaB_p2	TCCACTGCTCTACCAACTGA	TCATAAATGTTGCCCGTGGA	50.0	45.0	58.0	57.5	2314-2321 bp	0.8	75.9
dnaB_p3	TTCAGTCCACTGCTCTACCA	TCATAAATGTTGCCCGTGGA	50.0	45.0	58.0	57.5	2319-2326 bp	0.8	75.9
dnaB_p4	AGTCCACTGCTCTACCAACT	TCATAAATGTTGCCCGTGGA	50.0	45.0	58.0	57.5	2316-2323 bp	0.8	75.9
dnaB_p5	AGGTGGAGGAAGTTTAGGCT	TCATAAATGTTGCCCGTGGA	50.0	45.0	58.0	57.5	2898 bp	0.2	43.6