Hotspot

argB

Genome · 140263-141123 · 861 bp

Back to Lophurella

Pi 0.0633

MarkerSeek score 81.00

Primer available yes

Label argB

Feature Metadata

label_name: argB
feature_type: gene
region: Genome
length_bp: 861
variable_sites: 120
pi: 0.0633
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
argB_p1	GCAATCTGTCGCTTTCAACC	TAGCACCACCTGGATAACCA	50.0	50.0	58.0	58.0	1472-1475 bp	1.0	87.6
argB_p2	CGACCTTGGGCTTATGAGTC	TAGCACCACCTGGATAACCA	55.0	50.0	58.1	58.0	1392 bp	1.0	87.2
argB_p3	AGGGAGGTGCTCTAACCAAT	TAGCACCACCTGGATAACCA	50.0	50.0	58.0	58.0	1612-1615 bp	0.8	79.7
argB_p4	AGGGAGGTGCTCTAACCAAT	GCACCACCTGGATAACCAAA	50.0	50.0	58.0	58.1	1610-1613 bp	0.8	79.3
argB_p5	TACAGTCCCAATAGGCCACA	TAGCACCACCTGGATAACCA	50.0	50.0	58.0	58.0	1590 bp	0.2	47.4