Hotspot

QQP83_pgp116

Genome · 69393-70190 · 798 bp

Back to Lophurella

Pi 0.122

MarkerSeek score 79.60

Primer available yes

Label QQP83_pgp116

Feature Metadata

label_name: QQP83_pgp116
feature_type: gene
region: Genome
length_bp: 798
variable_sites: 209
pi: 0.122
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
QQP83_pgp116_p1	AGTCATTTGGCCGACTTGTT	CTCCATGGTTGGCAAGTTCT	45.0	50.0	58.0	58.1	1782-1790 bp	1.0	87.8
QQP83_pgp116_p2	AGTCATTTGGCCGACTTGTT	AACTCCATGGTTGGCAAGTT	45.0	45.0	58.0	57.9	1784-1792 bp	0.8	79.5
QQP83_pgp116_p3	AGTCATTTGGCCGACTTGTT	AGACCCATCGCTAAATGCTG	45.0	50.0	58.0	58.0	1913-1921 bp	0.6	72.1
QQP83_pgp116_p4	TCATTTGGCCGACTTGTTGA	AGACCCATCGCTAAATGCTG	45.0	50.0	58.0	58.0	1911-1919 bp	0.6	72.1
QQP83_pgp116_p5	GTCATTTGGCCGACTTGTTG	AGACCCATCGCTAAATGCTG	50.0	50.0	57.9	58.0	1912-1920 bp	0.6	71.9