Hotspot

trnY-trnV.2

Genome · 143346-143433 · 88 bp

Back to Lophurella

Pi 0.155

MarkerSeek score 81.00

Primer available yes

Label trnY-trnV.2

Feature Metadata

label_name: trnY-trnV.2
feature_type: igs
region: Genome
length_bp: 88
variable_sites: 26
pi: 0.155
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-trnV.2_p1	TTACAGTCCGCCCCCATTAA	ACACCGTAAAAGTCACTGGT	50.0	45.0	58.7	57.0	155-170 bp	1.0	78.3
trnY-trnV.2_p2	TTTACAGTCCGCCCCCATTA	ACACCGTAAAAGTCACTGGT	50.0	45.0	58.7	57.0	156-171 bp	1.0	78.3
trnY-trnV.2_p3	TTACAGTCCGCCCCCATTAA	GACACCGTAAAAGTCACTGGT	50.0	47.6	58.7	58.2	156-171 bp	1.0	76.9
trnY-trnV.2_p4	TTTACAGTCCGCCCCCATTA	GACACCGTAAAAGTCACTGGT	50.0	47.6	58.7	58.2	157-172 bp	1.0	76.9
trnY-trnV.2_p5	TTACAGTCCGCCCCCATTAA	ACACCGTAAAAGTCACTGGTT	50.0	42.9	58.7	57.7	155-170 bp	1.0	76.1