Hotspot

petN-acsF

Genome · 106084-106331 · 248 bp

Back to Lophurella

Pi 0.0747

MarkerSeek score 79.50

Primer available yes

Label petN-acsF

Feature Metadata

label_name: petN-acsF
feature_type: igs
region: Genome
length_bp: 248
variable_sites: 39
pi: 0.0747
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-acsF_p1	AAGGCTGCTGATCAAATGGA	ATAAACGCGATGTTCAGGGT	45.0	45.0	57.5	57.6	1423-1435 bp	0.8	72.1
petN-acsF_p2	AGGCTGCTGATCAAATGGAA	ATAAACGCGATGTTCAGGGT	45.0	45.0	57.5	57.6	1422-1434 bp	0.8	72.1
petN-acsF_p3	GGCTGCTGATCAAATGGAAC	ATAAACGCGATGTTCAGGGT	50.0	45.0	57.4	57.6	1421-1433 bp	0.8	71.6
petN-acsF_p4	AAGGCTGCTGATCAAATGGA	TAAACGCGATGTTCAGGGTG	45.0	50.0	57.5	58.6	1422-1434 bp	0.8	70.8
petN-acsF_p5	AGGCTGCTGATCAAATGGAA	TAAACGCGATGTTCAGGGTG	45.0	50.0	57.5	58.6	1421-1433 bp	0.8	70.8