primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf46_p1	ycf46	1	AAACCATTCCGAACTTGGCT	AATTAGACGGGGCCAAAGAC	20	20	45.0	50.0	58.0	57.9	1.3	4.4	0.128901	4788	6251	2230-2230	0.200000	47.0
ycf46_p2	ycf46	2	ATCTCCCCCAAGAGTTCACA	AATTAGACGGGGCCAAAGAC	20	20	50.0	50.0	58.0	57.9	0.0	4.4	0.169070	4788	6251	2728-2728	0.200000	46.6
ycf46_p3	ycf46	3	TCTCCCCCAAGAGTTCACAT	AATTAGACGGGGCCAAAGAC	20	20	50.0	50.0	58.0	57.9	0.0	4.4	0.169070	4788	6251	2727-2727	0.200000	46.6
ycf46_p4	ycf46	4	CAAGTCGAGCAGAGACGAAA	AATTAGACGGGGCCAAAGAC	20	20	50.0	50.0	57.9	57.9	0.0	4.4	0.176156	4788	6251	2828-2828	0.200000	46.6
ycf46_p5	ycf46	5	CAAACCATTCCGAACTTGGC	AATTAGACGGGGCCAAAGAC	20	20	50.0	50.0	57.9	57.9	1.3	4.4	0.188850	4788	6251	2231-2231	0.200000	46.5
ycf37_p1	ycf37	1	ACCACTTTTTGTTTTCATCCGA	TGGTTGGGTAAGGAGACCAT	22	20	36.4	50.0	57.0	57.9	0.0	0.0	3.046776	6541	7032	1233-1245	0.400000	37.7
ycf37_p2	ycf37	2	GACCACTTTTTGTTTTCATCCGA	TGGTTGGGTAAGGAGACCAT	23	20	39.1	50.0	58.1	57.9	0.0	0.0	3.126325	6541	7032	1234-1246	0.400000	37.1
ycf37_p3	ycf37	3	ACCACTTTTTGTTTTCATCCGA	TTGGTTGGGTAAGGAGACCA	22	20	36.4	50.0	57.0	58.2	0.0	0.0	3.170604	6541	7032	1234-1246	0.400000	36.7
ycf37_p4	ycf37	4	ACCACTTTTTGTTTTCATCCGA	GACTTTGTGACTCGGTTGGT	22	20	36.4	50.0	57.0	58.1	0.0	0.0	3.043564	6541	7032	1249-1249	0.200000	23.7
ycf37_p5	ycf37	5	GACCACTTTTTGTTTTCATCCGA	GACTTTGTGACTCGGTTGGT	23	20	39.1	50.0	58.1	58.1	0.0	0.0	3.123113	6541	7032	1250-1250	0.200000	23.0
accD_p1	accD	1	TGGAGCTGGTTTAACATGGG	TCTCTAGCAGGAGTCGCTAA	20	20	50.0	50.0	57.8	57.3	0.0	6.0	0.926354	10928	11797	1725-1736	1.000000	81.1
accD_p2	accD	2	ACTGCTCCTCATCCTAAGCT	TCTCTAGCAGGAGTCGCTAA	20	20	50.0	50.0	57.8	57.3	0.0	6.0	0.885104	10928	11797	2073-2073	0.200000	40.9
accD_p3	accD	3	ACTGCTCCTCATCCTAAGCT	CACCGTTATGACATTGAGCG	20	20	50.0	50.0	57.8	57.3	0.0	0.0	0.892451	10928	11797	2003-2003	0.200000	40.9
accD_p4	accD	4	TGGAGCTGGTTTAACATGGG	CACCGTTATGACATTGAGCG	20	20	50.0	50.0	57.8	57.3	0.0	0.0	0.933701	10928	11797	1656-1656	0.200000	40.5
accD_p5	accD	5	ACTGCTCCTCATCCTAAGCT	CGTTATGACATTGAGCGCAA	20	20	50.0	45.0	57.8	57.2	0.0	11.5	0.977546	10928	11797	2000-2000	0.200000	40.2
carA_p1	carA	1	AATGGTTATGGTCCGATGCC	GTGGACGATAGATGCACCAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.160652	13041	14210	1468-1489	0.600000	70.9
carA_p2	carA	2	AATGGTTATGGTCCGATGCC	GCACCAGAAGGACACTTACC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.209060	13041	14210	1455-1476	0.600000	70.4
carA_p3	carA	3	AATGGTTATGGTCCGATGCC	GATGCACCAGAAGGACACTT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.209871	13041	14210	1458-1479	0.600000	70.4
carA_p4	carA	4	AATGGTTATGGTCCGATGCC	TGGACGATAGATGCACCAGA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.243358	13041	14210	1467-1488	0.600000	70.2
carA_p5	carA	5	AATGGTTATGGTCCGATGCC	ATGTGGACGATAGATGCACC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.552010	13041	14210	1470-1491	0.600000	67.7
dsbD_p1	dsbD	1	ATCCTTCCTGGGGAGTTTCA	AGGAGCAATACGACCATACA	20	20	50.0	45.0	57.9	56.0	0.0	0.0	2.017457	23192	23929	1336-1336	0.200000	31.9
dsbD_p2	dsbD	2	TCAAATCCTTCCTGGGGAGT	AGGAGCAATACGACCATACA	20	20	50.0	45.0	57.9	56.0	0.0	0.0	2.017457	23192	23929	1340-1340	0.200000	31.9
dsbD_p3	dsbD	3	GGTTCAAATCCTTCCTGGGG	AGGAGCAATACGACCATACA	20	20	55.0	45.0	58.1	56.0	0.0	0.0	2.105068	23192	23929	1343-1343	0.200000	31.2
dsbD_p4	dsbD	4	TCCTTCCTGGGGAGTTTCAA	AGGAGCAATACGACCATACA	20	20	50.0	45.0	58.2	56.0	0.0	0.0	2.129909	23192	23929	1335-1335	0.200000	31.0
dsbD_p5	dsbD	5	TACCAATTTCACCACGTCCG	AGGAGCAATACGACCATACA	20	20	50.0	45.0	58.2	56.0	0.0	0.0	2.159417	23192	23929	1541-1541	0.200000	30.7
tilS_p1	tilS	1	AGGTCTTGCTCGACACAAAG	TATCAAGCATGAACAGCGCT	20	20	50.0	45.0	58.1	58.0	8.0	2.6	0.157340	32352	33335	1823-1823	0.200000	46.7
tilS_p2	tilS	2	GAAGGTCTTGCTCGACACAA	TATCAAGCATGAACAGCGCT	20	20	50.0	45.0	58.1	58.0	8.0	2.6	0.162555	32352	33335	1825-1825	0.200000	46.7
tilS_p3	tilS	3	GGTCTTGCTCGACACAAAGA	TATCAAGCATGAACAGCGCT	20	20	50.0	45.0	58.1	58.0	8.0	2.6	0.162555	32352	33335	1822-1822	0.200000	46.7
tilS_p4	tilS	4	TTGAAGGTCTTGCTCGACAC	TATCAAGCATGAACAGCGCT	20	20	50.0	45.0	58.1	58.0	8.0	2.6	0.162555	32352	33335	1827-1827	0.200000	46.7
tilS_p5	tilS	5	AGGTCTTGCTCGACACAAAG	AAGCATGAACAGCGCTTCTA	20	20	50.0	45.0	58.1	57.9	8.0	7.5	0.228061	32352	33335	1819-1819	0.200000	46.2
ycf23_p1	ycf23	1	AATGTGTTCGTGCTTGTCCT	ACATAAAAGCAGGCGACATCA	20	21	45.0	42.9	58.0	58.3	0.0	0.0	1.322305	46023	46871	1537-1537	0.200000	37.4
ycf23_p2	ycf23	2	ATGTGTTCGTGCTTGTCCTT	ACATAAAAGCAGGCGACATCA	20	21	45.0	42.9	58.0	58.3	0.0	0.0	1.322305	46023	46871	1536-1536	0.200000	37.4
ycf23_p3	ycf23	3	CAATGTGTTCGTGCTTGTCC	ACATAAAAGCAGGCGACATCA	20	21	50.0	42.9	58.0	58.3	0.0	0.0	1.326180	46023	46871	1538-1538	0.200000	37.4
ycf23_p4	ycf23	4	TCAAATAGCCTCCGCTCCTA	ACATAAAAGCAGGCGACATCA	20	21	50.0	42.9	57.9	58.3	0.0	0.0	1.371763	46023	46871	1473-1473	0.200000	37.0
ycf23_p5	ycf23	5	GACGGTTGTAAAGCTGGTCA	ACATAAAAGCAGGCGACATCA	20	21	50.0	42.9	58.1	58.3	0.0	0.0	1.415925	46023	46871	1490-1490	0.200000	36.7
ycf80_p1	ycf80	1	GGGTTCAAATCCCACCAGTT	AGGCGACTTACCAGGACTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.236644	54470	55984	2190-2190	0.200000	46.1
ycf80_p2	ycf80	2	GGGTTCAAATCCCACCAGTT	TTAGGCGACTTACCAGGACT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.236644	54470	55984	2192-2192	0.200000	46.1
ycf80_p3	ycf80	3	ACTTTGGTGGTCGAAGGTTC	AGGCGACTTACCAGGACTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.263790	54470	55984	2655-2655	0.200000	45.9
ycf80_p4	ycf80	4	ACTTTGGTGGTCGAAGGTTC	TTAGGCGACTTACCAGGACT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.263790	54470	55984	2657-2657	0.200000	45.9
ycf80_p5	ycf80	5	GACTTTGGTGGTCGAAGGTT	AGGCGACTTACCAGGACTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.263790	54470	55984	2656-2656	0.200000	45.9
ilvH_p1	ilvH	1	CTGCATTGTGCTGCTTCTTT	GATCGTGAGGGTTCAAGTCC	20	20	45.0	55.0	57.6	58.0	0.0	0.0	0.445409	57374	57910	1303-1303	0.200000	44.4
ilvH_p2	ilvH	2	CTGCATTGTGCTGCTTCTTT	AGGCGGTATAGCTAAGTGGT	20	20	45.0	50.0	57.6	57.9	0.0	0.0	0.517497	57374	57910	1443-1443	0.200000	43.9
ilvH_p3	ilvH	3	CTGCATTGTGCTGCTTCTTT	GGTAAGGCAGAGGATTGCAA	20	20	45.0	50.0	57.6	57.9	0.0	0.0	0.562639	57374	57910	1426-1426	0.200000	43.5
ilvH_p4	ilvH	4	CTGCATTGTGCTGCTTCTTT	CCCTCCATCCCCATTGACTA	20	20	45.0	55.0	57.6	58.2	0.0	0.0	0.614116	57374	57910	1285-1285	0.200000	43.1
ilvH_p5	ilvH	5	CTGCATTGTGCTGCTTCTTT	GCTAAGTGGTAAGGCAGAGG	20	20	45.0	55.0	57.6	57.7	0.0	0.0	0.751398	57374	57910	1433-1433	0.200000	42.0
trnM_p1	trnM	1	TTCCAAGCTAATGATGCGGG	TCACAAGAGAGCGCTAATGC	20	20	50.0	50.0	58.3	58.4	0.0	11.5	0.675827	64423	66321	2564-2594	0.600000	69.3
trnM_p2	trnM	2	GCTAATGATGCGGGTTCGAT	TCACAAGAGAGCGCTAATGC	20	20	50.0	50.0	58.5	58.4	0.0	11.5	0.842040	64423	66321	2558-2588	0.600000	68.0
trnM_p3	trnM	3	CCAAGCTAATGATGCGGGTT	TCACAAGAGAGCGCTAATGC	20	20	50.0	50.0	58.6	58.4	0.0	11.5	0.962377	64423	66321	2562-2592	0.600000	67.0
trnM_p4	trnM	4	TTACCCCAAGGAGCAGCTAT	TCTCCTTCTGCTGTTGCTAC	20	20	50.0	50.0	58.1	57.2	0.0	0.0	0.857851	64423	66321	2680-2699	0.400000	58.2
trnM_p5	trnM	5	TTACCCCAAGGAGCAGCTAT	CTCCTTCTGCTGTTGCTACT	20	20	50.0	50.0	58.1	57.2	0.0	0.0	0.865421	64423	66321	2679-2698	0.400000	58.1
trnM-cemA_p1	trnM-cemA	1	TAAAAACGGGTCGCACATGA	TCTCCTTCTGCTGTTGCTAC	20	20	45.0	50.0	58.1	57.2	0.0	0.0	0.870895	66322	66508	283-408	1.000000	85.0
trnM-cemA_p2	trnM-cemA	2	TAAAAACGGGTCGCACATGA	CTCCTTCTGCTGTTGCTACT	20	20	45.0	50.0	58.1	57.2	0.0	0.0	0.878465	66322	66508	282-407	1.000000	85.0
trnM-cemA_p3	trnM-cemA	3	CTAAAAACGGGTCGCACATG	TCTCCTTCTGCTGTTGCTAC	20	20	50.0	50.0	57.8	57.2	0.0	0.0	0.992426	66322	66508	284-409	1.000000	84.1
trnM-cemA_p4	trnM-cemA	4	CTAAAAACGGGTCGCACATG	CTCCTTCTGCTGTTGCTACT	20	20	50.0	50.0	57.8	57.2	0.0	0.0	0.999995	66322	66508	283-408	1.000000	84.0
trnM-cemA_p5	trnM-cemA	5	TAAAAACGGGTCGCACATGA	CTCCTTCTGCTGTTGCTACTT	20	21	45.0	47.6	58.1	57.9	0.0	0.0	1.170810	66322	66508	282-407	1.000000	82.6
ycf58_p1	ycf58	1	GCATTAGCGCTCTCTTGTGA	ATCAGGTCGTCGTGCTAAAA	20	20	50.0	45.0	58.4	57.3	16.3	0.0	1.091110	67479	67892	1614-1656	0.600000	63.4
ycf58_p2	ycf58	2	GCATTAGCGCTCTCTTGTGA	TCAGGTCGTCGTGCTAAAAT	20	20	50.0	45.0	58.4	57.3	16.3	0.0	1.091110	67479	67892	1613-1655	0.600000	63.4
ycf58_p3	ycf58	3	GCATTAGCGCTCTCTTGTGA	ATTGAAATGTTAGCGCGTGG	20	20	50.0	45.0	58.4	57.5	16.3	8.4	0.897732	67479	67892	1502-1502	0.200000	40.8
ycf58_p4	ycf58	4	GCATTAGCGCTCTCTTGTGA	TGAAATGTTAGCGCGTGGTT	20	20	50.0	45.0	58.4	58.8	16.3	8.4	1.123745	67479	67892	1500-1500	0.200000	39.0
ycf58_p5	ycf58	5	GCATTAGCGCTCTCTTGTGA	TTGAAATGTTAGCGCGTGGT	20	20	50.0	45.0	58.4	58.8	16.3	8.4	1.123745	67479	67892	1501-1501	0.200000	39.0
QQP83_pgp116_p1	QQP83_pgp116	1	AGTCATTTGGCCGACTTGTT	CTCCATGGTTGGCAAGTTCT	20	20	45.0	50.0	58.0	58.1	5.3	12.6	0.103603	69393	70190	1782-1790	1.000000	87.8
QQP83_pgp116_p2	QQP83_pgp116	2	AGTCATTTGGCCGACTTGTT	AACTCCATGGTTGGCAAGTT	20	20	45.0	45.0	58.0	57.9	5.3	12.6	0.105354	69393	70190	1784-1792	0.800000	79.5
QQP83_pgp116_p3	QQP83_pgp116	3	AGTCATTTGGCCGACTTGTT	AGACCCATCGCTAAATGCTG	20	20	45.0	50.0	58.0	58.0	5.3	0.0	0.059702	69393	70190	1913-1921	0.600000	72.1
QQP83_pgp116_p4	QQP83_pgp116	4	TCATTTGGCCGACTTGTTGA	AGACCCATCGCTAAATGCTG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.066693	69393	70190	1911-1919	0.600000	72.1
QQP83_pgp116_p5	QQP83_pgp116	5	GTCATTTGGCCGACTTGTTG	AGACCCATCGCTAAATGCTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.091435	69393	70190	1912-1920	0.600000	71.9
petN-acsF_p1	petN-acsF	1	AAGGCTGCTGATCAAATGGA	ATAAACGCGATGTTCAGGGT	20	20	45.0	45.0	57.5	57.6	0.0	15.9	0.916361	106084	106331	1423-1435	0.800000	72.1
petN-acsF_p2	petN-acsF	2	AGGCTGCTGATCAAATGGAA	ATAAACGCGATGTTCAGGGT	20	20	45.0	45.0	57.5	57.6	0.0	15.9	0.916361	106084	106331	1422-1434	0.800000	72.1
petN-acsF_p3	petN-acsF	3	GGCTGCTGATCAAATGGAAC	ATAAACGCGATGTTCAGGGT	20	20	50.0	45.0	57.4	57.6	0.0	15.9	0.970531	106084	106331	1421-1433	0.800000	71.6
petN-acsF_p4	petN-acsF	4	AAGGCTGCTGATCAAATGGA	TAAACGCGATGTTCAGGGTG	20	20	45.0	50.0	57.5	58.6	0.0	15.9	1.085216	106084	106331	1422-1434	0.800000	70.8
petN-acsF_p5	petN-acsF	5	AGGCTGCTGATCAAATGGAA	TAAACGCGATGTTCAGGGTG	20	20	45.0	50.0	57.5	58.6	0.0	15.9	1.085216	106084	106331	1421-1433	0.800000	70.8
argB_p1	argB	1	GCAATCTGTCGCTTTCAACC	TAGCACCACCTGGATAACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.071187	140263	141123	1472-1475	1.000000	87.6
argB_p2	argB	2	CGACCTTGGGCTTATGAGTC	TAGCACCACCTGGATAACCA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.098965	140263	141123	1392-1392	1.000000	87.2
argB_p3	argB	3	AGGGAGGTGCTCTAACCAAT	TAGCACCACCTGGATAACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067703	140263	141123	1612-1615	0.800000	79.7
argB_p4	argB	4	AGGGAGGTGCTCTAACCAAT	GCACCACCTGGATAACCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.109540	140263	141123	1610-1613	0.800000	79.3
argB_p5	argB	5	TACAGTCCCAATAGGCCACA	TAGCACCACCTGGATAACCA	20	20	50.0	50.0	58.0	58.0	5.0	0.0	0.080581	140263	141123	1590-1590	0.200000	47.4
trnY-trnV.2_p1	trnY-trnV.2	1	TTACAGTCCGCCCCCATTAA	ACACCGTAAAAGTCACTGGT	20	20	50.0	45.0	58.7	57.0	0.0	0.0	1.706968	143346	143433	155-170	1.000000	78.3
trnY-trnV.2_p2	trnY-trnV.2	2	TTTACAGTCCGCCCCCATTA	ACACCGTAAAAGTCACTGGT	20	20	50.0	45.0	58.7	57.0	0.0	0.0	1.706968	143346	143433	156-171	1.000000	78.3
trnY-trnV.2_p3	trnY-trnV.2	3	TTACAGTCCGCCCCCATTAA	GACACCGTAAAAGTCACTGGT	20	21	50.0	47.6	58.7	58.2	0.0	0.0	1.882678	143346	143433	156-171	1.000000	76.9
trnY-trnV.2_p4	trnY-trnV.2	4	TTTACAGTCCGCCCCCATTA	GACACCGTAAAAGTCACTGGT	20	21	50.0	47.6	58.7	58.2	0.0	0.0	1.882678	143346	143433	157-172	1.000000	76.9
trnY-trnV.2_p5	trnY-trnV.2	5	TTACAGTCCGCCCCCATTAA	ACACCGTAAAAGTCACTGGTT	20	21	50.0	42.9	58.7	57.7	0.0	0.0	1.983838	143346	143433	155-170	1.000000	76.1
dnaB_p1	dnaB	1	TCAGTCCACTGCTCTACCAA	TCATAAATGTTGCCCGTGGA	20	20	50.0	45.0	58.0	57.5	1.5	0.0	0.508326	146719	148536	2318-2325	0.800000	75.9
dnaB_p2	dnaB	2	TCCACTGCTCTACCAACTGA	TCATAAATGTTGCCCGTGGA	20	20	50.0	45.0	58.0	57.5	0.0	0.0	0.508326	146719	148536	2314-2321	0.800000	75.9
dnaB_p3	dnaB	3	TTCAGTCCACTGCTCTACCA	TCATAAATGTTGCCCGTGGA	20	20	50.0	45.0	58.0	57.5	1.5	0.0	0.508326	146719	148536	2319-2326	0.800000	75.9
dnaB_p4	dnaB	4	AGTCCACTGCTCTACCAACT	TCATAAATGTTGCCCGTGGA	20	20	50.0	45.0	58.0	57.5	0.0	0.0	0.515549	146719	148536	2316-2323	0.800000	75.9
dnaB_p5	dnaB	5	AGGTGGAGGAAGTTTAGGCT	TCATAAATGTTGCCCGTGGA	20	20	50.0	45.0	58.0	57.5	0.0	0.0	0.544924	146719	148536	2898-2898	0.200000	43.6
ycf29_p1	ycf29	1	TGTTGATTCTTTACTTGGCCA	AGGCAATGGATTGTGACTCC	21	20	38.1	50.0	55.7	57.9	0.0	1.5	3.444059	154019	154639	1201-1201	0.600000	43.4
ycf29_p2	ycf29	2	TGTTGATTCTTTACTTGGCCA	GGCAATGGATTGTGACTCCA	21	20	38.1	50.0	55.7	58.2	0.0	0.0	3.471158	154019	154639	1200-1200	0.600000	43.1
ycf29_p3	ycf29	3	TGTTGATTCTTTACTTGGCCAA	AGGCAATGGATTGTGACTCC	22	20	36.4	50.0	56.4	57.9	19.3	1.5	3.686977	154019	154639	1201-1201	0.600000	41.4
ycf29_p4	ycf29	4	TTGTTGATTCTTTACTTGGCCA	AGGCAATGGATTGTGACTCC	22	20	36.4	50.0	56.4	57.9	0.0	1.5	3.686977	154019	154639	1202-1202	0.600000	41.4
ycf29_p5	ycf29	5	TGTTGATTCTTTACTTGGCCAA	GGCAATGGATTGTGACTCCA	22	20	36.4	50.0	56.4	58.2	19.3	0.0	3.714076	154019	154639	1200-1200	0.600000	41.2
syfB_p1	syfB	1	TGCCACAGAAATATACCGCC	CGAATCCTCCTACCCCAGAT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.055111	156265	158283	2773-2781	1.000000	87.8
syfB_p2	syfB	2	TCGGTAAGAGCGTACCAGAT	CGAATCCTCCTACCCCAGAT	20	20	50.0	55.0	58.0	58.0	12.0	0.0	0.065349	156265	158283	2725-2733	1.000000	87.7
syfB_p3	syfB	3	GTTCGGTAAGAGCGTACCAG	CGAATCCTCCTACCCCAGAT	20	20	55.0	55.0	58.1	58.0	12.0	0.0	0.111455	156265	158283	2727-2735	1.000000	87.4
syfB_p4	syfB	4	TGCCACAGAAATATACCGCC	GATCACGTGACGGTAGTACG	20	20	50.0	55.0	58.0	57.9	0.0	22.7	0.120939	156265	158283	2641-2641	0.200000	47.0
syfB_p5	syfB	5	TCGGTAAGAGCGTACCAGAT	GATCACGTGACGGTAGTACG	20	20	50.0	55.0	58.0	57.9	12.0	22.7	0.131177	156265	158283	2593-2593	0.200000	47.0