Hotspot

rps18

Genome · 28045-29376 · 1332 bp

Back to Lophophora

Pi 0

MarkerSeek score 63.00

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: Genome
length_bp: 1332
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TGTCCAACCGAAATAGGATT	TGAAAACAAAACAGCAATCCA	40.0	33.3	54.3	55.0	1388-1451 bp	1.0	44.0
rps18_p2	TGTCCAACCGAAATAGGATTT	TGAAAACAAAACAGCAATCCA	38.1	33.3	55.2	55.0	1388-1451 bp	1.0	44.0
rps18_p3	TGTCCAACCGAAATAGGATT	TTGAAAACAAAACAGCAATCCA	40.0	31.8	54.3	55.8	1389-1452 bp	1.0	44.0
rps18_p4	TGTCCAACCGAAATAGGATTTTC	TGAAAACAAAACAGCAATCCA	39.1	33.3	57.1	55.0	1388-1451 bp	1.0	44.0
rps18_p5	ATGTCCAACCGAAATAGGAT	TGAAAACAAAACAGCAATCCA	40.0	33.3	54.1	55.0	1389-1452 bp	1.0	44.0