Hotspot

psaC-rps15

Genome · 102018-102776 · 759 bp

Back to Lophophora

Pi 0

MarkerSeek score 58.90

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: Genome
length_bp: 759
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	GCCTTACATCCATCCCAAGG	GGCAACGACTACTGGCTTAT	55.0	50.0	58.0	57.7	954-955 bp	1.0	77.5
psaC-rps15_p2	ACATCTGTAGGACAAGCTCG	GGCAACGACTACTGGCTTAT	50.0	50.0	57.1	57.7	921-922 bp	1.0	70.0
psaC-rps15_p3	CATCTGTAGGACAAGCTCGT	GGCAACGACTACTGGCTTAT	50.0	50.0	57.1	57.7	920-921 bp	1.0	70.0
psaC-rps15_p4	GTAGGACAAGCTCGTACACA	GGCAACGACTACTGGCTTAT	50.0	50.0	57.0	57.7	915-916 bp	1.0	69.6
psaC-rps15_p5	TGTAGGACAAGCTCGTACAC	GGCAACGACTACTGGCTTAT	50.0	50.0	57.0	57.7	916-917 bp	1.0	69.6