Hotspot

rrn4.5-rrn5

Genome · 92809-93650 · 842 bp

Back to Lophophora

Pi 0.011

MarkerSeek score 67.90

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: Genome
length_bp: 842
variable_sites: 9
pi: 0.011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	CCTCTACGCCTAGTACACCA	55.0	55.0	58.0	58.0	903-930 bp	1.0	82.2
rrn4.5-rrn5_p2	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	959-986 bp	1.0	82.1
rrn4.5-rrn5_p3	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	957-984 bp	1.0	82.1
rrn4.5-rrn5_p4	GCTGAGGCATCCTAACAGAC	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.1	58.0	957-984 bp	1.0	82.1
rrn4.5-rrn5_p5	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	955-982 bp	1.0	82.1