Hotspot

trnI-CAU-rps7

Genome · 81707-84040 · 2334 bp

Back to Lophophora

Pi 0

MarkerSeek score 58.40

Primer available yes

Label trnI-CAU-rps7

Feature Metadata

label_name: trnI-CAU-rps7
feature_type: igs
region: Genome
length_bp: 2334
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rps7_p1	GTTGGGCGCTTTAACCATTC	CTTTGGCACATTTTCGTCGT	50.0	45.0	58.0	57.6	2355-2389 bp	1.0	77.9
trnI-CAU-rps7_p2	GTTGGGCGCTTTAACCATTC	AGCTTTGGCACATTTTCGTC	50.0	45.0	58.0	57.6	2357-2391 bp	1.0	77.7
trnI-CAU-rps7_p3	GTTGGGCGCTTTAACCATTC	GAGCTTTGGCACATTTTCGT	50.0	45.0	58.0	57.6	2358-2392 bp	1.0	77.7
trnI-CAU-rps7_p4	GACTTATGTCCCATTGGCGT	CTTTGGCACATTTTCGTCGT	50.0	45.0	58.0	57.6	2416-2450 bp	1.0	77.6
trnI-CAU-rps7_p5	GACTTATGTCCCATTGGCGT	AGCTTTGGCACATTTTCGTC	50.0	45.0	58.0	57.6	2418-2452 bp	1.0	77.4