Hotspot

trnS-UGA-psbC

Genome · 40487-40741 · 255 bp

Back to Lophophora

Pi 0

MarkerSeek score 52.90

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 255
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	CCTGTTCTTTCGATGACCCC	55.0	55.0	58.0	58.3	372-373 bp	1.0	78.0
trnS-UGA-psbC_p2	GTTGGAGAGATGGCTGAGTG	CCTGTTCTTTCGATGACCCC	55.0	55.0	58.0	58.3	376-377 bp	1.0	78.0
trnS-UGA-psbC_p3	GCTGAGTGGTTGATAGCTCC	CCTGTTCTTTCGATGACCCC	55.0	55.0	58.1	58.3	364-365 bp	1.0	77.7
trnS-UGA-psbC_p4	GGCTGAGTGGTTGATAGCTC	CCTGTTCTTTCGATGACCCC	55.0	55.0	58.1	58.3	365-366 bp	1.0	77.7
trnS-UGA-psbC_p5	GGTTGATAGCTCCGGTCTTG	CCTGTTCTTTCGATGACCCC	55.0	55.0	58.1	58.3	357-358 bp	1.0	77.7