Hotspot

trnM-CAU-rps14

Genome · 32420-38618 · 6199 bp

Back to Lophophora

Pi 0.000161
MarkerSeek score 52.60
Primer available yes
Label trnM-CAU-rps14

Feature Metadata

label_name
trnM-CAU-rps14
feature_type
igs
region
Genome
length_bp
6199
variable_sites
1
pi
0.000161
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-rps14_p1 GGGCACTTGGAATCCTATGG CCACCTCCTTACGAATCGTG 55.0 55.0 58.0 58.1 227 bp 1.0 79.6
trnM-CAU-rps14_p2 TCAGGGCACTTGGAATCCTA CCACCTCCTTACGAATCGTG 50.0 55.0 58.0 58.1 230 bp 1.0 79.5
trnM-CAU-rps14_p3 GTTGATCAGGGCACTTGGAA CCACCTCCTTACGAATCGTG 50.0 55.0 58.1 58.1 235 bp 1.0 79.0
trnM-CAU-rps14_p4 GGGCACTTGGAATCCTATGG TCCACCTCCTTACGAATCGT 55.0 50.0 58.0 58.2 228 bp 1.0 79.0
trnM-CAU-rps14_p5 GGGCACTTGGAATCCTATGG CGTGTCTTCATCAACCCAGT 55.0 50.0 58.0 57.8 211 bp 1.0 78.9