Hotspot

psaA-ycf3

Genome · 17006-17959 · 954 bp

Back to Lophophora

Pi 0

MarkerSeek score 59.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: Genome
length_bp: 954
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	CGATAACGCTTACTCCCGAG	50.0	55.0	58.1	58.0	1106-1110 bp	1.0	79.4
psaA-ycf3_p2	GTTCTTGAAAAATGGCCCGG	CGATAACGCTTACTCCCGAG	50.0	55.0	57.9	58.0	1125-1129 bp	1.0	79.4
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	1133-1137 bp	1.0	79.3
psaA-ycf3_p4	GTTCTTGAAAAATGGCCCGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1152-1156 bp	1.0	79.3
psaA-ycf3_p5	GTCTGGCCCATTCCTCAAAA	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	1135-1139 bp	1.0	78.5