Hotspot

trnK-UUU-rpl20

Genome · 4260-4896 · 637 bp

Back to Lophophora

Pi 0

MarkerSeek score 58.90

Primer available yes

Label trnK-UUU-rpl20

Feature Metadata

label_name: trnK-UUU-rpl20
feature_type: igs
region: Genome
length_bp: 637
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rpl20_p1	AAAGCCGAGTACTCTACCGT	ATTTCAATCAAGTCCCCCGG	50.0	50.0	58.2	57.9	697-698 bp	1.0	77.6
trnK-UUU-rpl20_p2	AAGCCGAGTACTCTACCGTT	ATTTCAATCAAGTCCCCCGG	50.0	50.0	58.2	57.9	696-697 bp	1.0	77.6
trnK-UUU-rpl20_p3	TCCTAGCCGCACTTAAAAGC	ATTTCAATCAAGTCCCCCGG	50.0	50.0	58.3	57.9	712-713 bp	1.0	76.8
trnK-UUU-rpl20_p4	AAAGCCGAGTACTCTACCGT	TCAAGTCCCCCGGAGAATAA	50.0	50.0	58.2	57.7	690-691 bp	1.0	76.7
trnK-UUU-rpl20_p5	AAGCCGAGTACTCTACCGTT	TCAAGTCCCCCGGAGAATAA	50.0	50.0	58.2	57.7	689-690 bp	1.0	76.7