Hotspot

trnH-GUG-psbA

Genome · 81-370 · 290 bp

Back to Lophophora

Pi 0

MarkerSeek score 33.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: Genome
length_bp: 290
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CCTTCAACCACTTGGCTACA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	364 bp	1.0	77.2
trnH-GUG-psbA_p2	TCAACCACTTGGCTACATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.5	57.9	361 bp	1.0	75.5
trnH-GUG-psbA_p3	CCTTCAACCACTTGGCTACA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.7	58.3	413 bp	1.0	75.1
trnH-GUG-psbA_p4	CCTTCAACCACTTGGCTACA	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.7	57.6	414 bp	1.0	74.7
trnH-GUG-psbA_p5	TCAACCACTTGGCTACATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.5	58.3	410 bp	1.0	73.4