Hotspot

rps16

LSC · 5766-6702 · 937 bp

Back to Lophira

Pi 0.00214
MarkerSeek score 60.10
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
937
variable_sites
2
pi
0.00214
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACAGTTCTTGTGGGTTGAGC ACCGGTTCAAAACTCAAGGA 50.0 45.0 58.3 57.3 1034-1035 bp 1.0 71.9
rps16_p2 AGTTCTTGTGGGTTGAGCAC ACCGGTTCAAAACTCAAGGA 50.0 45.0 58.3 57.3 1032-1033 bp 1.0 71.9
rps16_p3 GTTCTTGTGGGTTGAGCACT ACCGGTTCAAAACTCAAGGA 50.0 45.0 58.3 57.3 1031-1032 bp 1.0 71.9
rps16_p4 CAGTTCTTGTGGGTTGAGCA ACCGGTTCAAAACTCAAGGA 50.0 45.0 58.3 57.3 1033-1034 bp 1.0 71.9
rps16_p5 TCTTGTGGGTTGAGCACTTT ACCGGTTCAAAACTCAAGGA 45.0 45.0 57.6 57.3 1029-1030 bp 1.0 71.0