Hotspot

trnN-trnR

IRa · 134761-135387 · 627 bp

Back to Lophira

Pi 0

MarkerSeek score 60.40

Primer available yes

Label trnN-trnR

Feature Metadata

label_name: trnN-trnR
feature_type: igs
region: IRa
length_bp: 627
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-trnR_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	757-768 bp	1.0	79.4
trnN-trnR_p2	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	721-732 bp	1.0	78.3
trnN-trnR_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	708-719 bp	1.0	78.3
trnN-trnR_p4	CTGGTCGTAGGTTCGAATCC	CAGAGGATTAGAGCACGTGG	55.0	55.0	57.8	57.8	709-720 bp	1.0	77.9
trnN-trnR_p5	CCGTTGTTCCTCAGTAGCTC	ACAGATCCAGTGGAGACAGG	55.0	55.0	58.0	58.4	792-803 bp	1.0	77.6