Hotspot

ccsA-ndhD

SSC · 120091-120444 · 354 bp

Back to Lophira

Pi 0.00301

MarkerSeek score 59.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 354
variable_sites: 1
pi: 0.00301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAATTCCGCAATTGTCGCCT	GTCAGTCGACAAGGTTGAAGA	45.0	47.6	58.3	57.9	499-521 bp	1.0	72.8
ccsA-ndhD_p2	TTAATTCCGCAATTGTCGCC	GTCAGTCGACAAGGTTGAAGA	45.0	47.6	57.4	57.9	500-522 bp	1.0	70.4
ccsA-ndhD_p3	TAATTCCGCAATTGTCGCCT	TCAGTCGACAAGGTTGAAGA	45.0	45.0	58.3	56.4	498-520 bp	1.0	69.1
ccsA-ndhD_p4	TAATTCCGCAATTGTCGCCT	GTCAGTCGACAAGGTTGAAG	45.0	50.0	58.3	56.4	499-521 bp	1.0	68.9
ccsA-ndhD_p5	CCGCAATTGTCGCCTCTAT	GTCAGTCGACAAGGTTGAAGA	52.6	47.6	58.0	57.9	494-516 bp	1.0	66.8