Hotspot

trnR-trnN

IRb · 112635-113261 · 627 bp

Back to Lophira

Pi 0

MarkerSeek score 60.40

Primer available yes

Label trnR-trnN

Feature Metadata

label_name: trnR-trnN
feature_type: igs
region: IRb
length_bp: 627
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnN_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	757-768 bp	1.0	79.4
trnR-trnN_p2	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	721-732 bp	1.0	78.3
trnR-trnN_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	708-719 bp	1.0	78.3
trnR-trnN_p4	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	709-720 bp	1.0	77.9
trnR-trnN_p5	ACAGATCCAGTGGAGACAGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.4	58.0	792-803 bp	1.0	77.6