Hotspot

rps19-rpl2

IRb · 88419-88483 · 65 bp

Back to Lophira

Pi 0.0154

MarkerSeek score 61.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 65
variable_sites: 1
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	CGGGACCGCGTTACTATTAT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	57.3	58.1	291-292 bp	1.0	74.1
rps19-rpl2_p2	CGGGACCGCGTTACTATTAT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	57.3	58.1	199-200 bp	1.0	73.9
rps19-rpl2_p3	CGGGACCGCGTTACTATTAT	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.3	57.8	257-258 bp	1.0	72.9
rps19-rpl2_p4	CGGGACCGCGTTACTATTAT	ATTTGATTCTTCGTCGCCGT	50.0	45.0	57.3	58.3	201-202 bp	1.0	72.5
rps19-rpl2_p5	CGGGACCGCGTTACTATTAT	TTTGATTCTTCGTCGCCGTA	50.0	45.0	57.3	57.6	200-201 bp	1.0	71.9