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rps8-rpl14

LSC · 84607-84841 · 235 bp

Back to Lophira

Pi 0.0128

MarkerSeek score 59.80

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 235
variable_sites: 3
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATAGTGTCCCTACCCA	CGATTGCCCGAGAATTGAGA	50.0	50.0	57.7	58.1	324 bp	1.0	78.1
rps8-rpl14_p2	AGCAATAGTGTCCCTACCCA	TTGCCCGAGAATTGAGACAG	50.0	50.0	57.7	57.9	321 bp	1.0	77.8
rps8-rpl14_p3	AGCAATAGTGTCCCTACCCA	GCCCGAGAATTGAGACAGTT	50.0	50.0	57.7	57.9	319 bp	1.0	77.8
rps8-rpl14_p4	AGCAATAGTGTCCCTACCCA	AATTTTTGGTGCGATTGCCC	50.0	45.0	57.7	58.2	335 bp	1.0	77.1
rps8-rpl14_p5	CAGCAATAGTGTCCCTACCC	CGATTGCCCGAGAATTGAGA	55.0	50.0	57.4	58.1	325 bp	1.0	75.3