Hotspot

rbcL-accD

LSC · 60552-61445 · 894 bp

Back to Lophira

Pi 0.00224
MarkerSeek score 60.00
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
894
variable_sites
2
pi
0.00224
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGGCTAGCAAATGGAGTCC AGCATCGAATTGAACCACCA 55.0 45.0 58.0 57.8 1013-1014 bp 1.0 78.3
rbcL-accD_p2 GAGTCCTGAACTAGCTGCTG AGCATCGAATTGAACCACCA 55.0 45.0 57.7 57.8 999-1000 bp 1.0 76.2
rbcL-accD_p3 GGCTAGCAAATGGAGTCCTG AGCATCGAATTGAACCACCA 55.0 45.0 58.3 57.8 1011-1012 bp 1.0 76.0
rbcL-accD_p4 AGGCTAGCAAATGGAGTCCT AGCATCGAATTGAACCACCA 50.0 45.0 58.4 57.8 1012-1013 bp 1.0 75.3
rbcL-accD_p5 CGAGGCTAGCAAATGGAGTC AGCATCGAATTGAACCACCA 55.0 45.0 58.4 57.8 1014-1015 bp 1.0 75.2