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petA-psbJ

LSC · 65988-66997 · 1010 bp

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Pi 0.0151

MarkerSeek score 68.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 156
pi: 0.0151
unique_haplotype_count: 64
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCG	50.0	50.0	56.8	58.0	1047-1105 bp	0.9875	81.9
petA-psbJ_p2	CGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCG	47.6	50.0	58.3	58.0	1047-1105 bp	0.9875	80.7
petA-psbJ_p3	TCGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCG	47.6	50.0	58.3	58.0	1048-1106 bp	0.9875	80.7
petA-psbJ_p4	CGAGAAGGTTCAATTGTCCG	GGATTGGGTTCATCGCTGTA	50.0	50.0	56.8	57.7	1042-1100 bp	0.9875	79.6
petA-psbJ_p5	CGAGAAGGTTCAATTGTCCG	AGCTGGGATTCTTGTGATCG	50.0	50.0	56.8	57.7	1097-1155 bp	0.975	79.1