Hotspot

trnE-UUC-trnT-GGU

LSC · 32981-33760 · 780 bp

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Pi 0.0231

MarkerSeek score 73.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 135
pi: 0.0231
unique_haplotype_count: 64
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGATCAACTCCCTTTCCCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	545-1036 bp	1.0	91.7
trnE-UUC-trnT-GGU_p2	CGATCAACTCCCTTTCCCAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.0	58.2	542-1033 bp	1.0	90.6
trnE-UUC-trnT-GGU_p3	CGATCAACTCCCTTTCCCAG	ACTCTACCGCTGACTGAGTT	55.0	50.0	58.0	58.1	514-1005 bp	0.9875	90.6
trnE-UUC-trnT-GGU_p4	TCCCTTTCCCAGTACCCTAC	GAACCGATGACTTACGCCTT	55.0	50.0	57.7	58.0	537-1028 bp	1.0	89.9
trnE-UUC-trnT-GGU_p5	CGATCAACTCCCTTTCCCAG	CGTTACTCTACCGCTGACTG	55.0	55.0	58.0	57.8	518-1009 bp	0.9875	89.9