Hotspot

trnK-UUU-trnQ-UUG

LSC · 4299-7726 · 3428 bp

Back to Lonicera

Pi 0.014

MarkerSeek score 68.70

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 3428
variable_sites: 458
pi: 0.014
unique_haplotype_count: 76
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	ACTTGTGTTGGATTGGCACT	GCGGAGGCTTTTAAGGAACT	45.0	50.0	57.9	58.2	171-181 bp	1.0	90.0
trnK-UUU-trnQ-UUG_p2	GACTTGTGTTGGATTGGCAC	GCGGAGGCTTTTAAGGAACT	50.0	50.0	57.6	58.2	172-182 bp	1.0	87.2
trnK-UUU-trnQ-UUG_p3	AGACTTGTGTTGGATTGGCA	GCGGAGGCTTTTAAGGAACT	45.0	50.0	57.6	58.2	173-183 bp	0.9875	87.2
trnK-UUU-trnQ-UUG_p4	TGTGTTGGATTGGCACTACA	GCGGAGGCTTTTAAGGAACT	45.0	50.0	57.4	58.2	168-178 bp	1.0	85.4
trnK-UUU-trnQ-UUG_p5	TTGTGTTGGATTGGCACTACA	GCGGAGGCTTTTAAGGAACT	42.9	50.0	58.1	58.2	169-179 bp	1.0	82.1